pKa and LogP Determination Assay Service

SiriusT3: determination of pKa, logP/D and solubility analytical instrument.

What is pKa and LogP Analysis and why is it important?

Our pKa and LogP analysis services offer accurate assessments of compound properties crucial for understanding their behaviour in various environments. pKa analysis determines the acidity or basicity of a molecule, while Log P analysis evaluates its lipophilicity or hydrophilicity

What is pKa and LogP Analysis used for?

pKa and LogP is particularly adept at providing insights into:

  • Solubility of Ionizable Compounds
  • Assessing Lipophilicity
  • Predicting Pharmacokinetic Properties
  • Informing Drug Design and Optimization
  • Understanding Ionization Behavior
  • Assessing Chemical Reactivity
  • Evaluating Drug Absorption and Distribution
  • Chemical Process Optimization
  • Analysing Acidity/Basicity

Our pKa and LogP Services

Advanced Drug Delivery Systems:

Incorporating materials with defined pKa values is instrumental in crafting drug delivery systems with controlled release kinetics.

Surface Engineering:

Surface modification techniques rely on precise control over pKa values to regulate interactions with surrounding environments.

Tailoring Material Properties:

Inspecting drug powders for uniformity, purity, and the presence of any foreign particles.

Sensing and Analytical Applications

Materials with tailored pKa values serve as building blocks for sensitive sensors and biosensors.

Our pKa and LogP Analysis Process

1

Choose Your Method

Select the appropriate method for your pKa and logP determination:

Fast UV titration for pKa
UV-metric titration for pKa
Potentiometric (pH-metric) titration for pKa and log P

2

We utilise our necessary materials and quantities for accurate testing to prepare your samples:

For UV-metric titration: Prepare 3-5 µL of 10mM stock solution.
For pH-metric titration: Provide 2-5mg of solid compound.

3

Experimentation:

We utilise our advanced SiriusT3 instrument programmed with suitable assay parameters for analysis. 

4

Receive comprehensive data delivery, including:

pKa values
Optional log P determination
Standard error analysis
Calculated log D at pH 7.4 based on pKa and log P

Advantages of Our pKa and LogP Analysis Services

  1. State-of-the-Art Equipment: Employment of cutting-edge siriuT3 technology for reliable results.

  2. Expertise: Our team comprises experienced professionals in analytical chemistry and compound property assessment.

  3. Customised Solutions: Tailored services to meet specific research and development needs.

  4. Comprehensive data reporting: Actionable Insights: Detailed reports providing actionable insights for decision-making.

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Frequently Asked Questions

LogP indicates the hydrophobicity or lipophilicity of a compound.

A good LogP value varies depending on how it’s used. For drug design, it’s important for LogP to enable effective absorption and distribution across biological membranes while keeping enough water solubility. But there isn’t a one-size-fits-all “good” LogP value. It depends on factors like the biological system, how the drug is given, and the drug’s specific properties. Generally, drug candidates with LogP values within a certain range for their purpose are seen as favorable.

pKa measures how acidic a compound is, while LogP tells us how hydrophobic it is. Compounds with lower pKa values act as stronger acids, making them more soluble in water and reducing LogP. Conversely, compounds with higher pKa values are weaker acids, leading to decreased water solubility and higher LogP. 

Achieving the right balance between pKa and LogP is crucial in drug design, ensuring effective absorption and distribution across biological membranes while maintaining solubility. In the field of medicinal chemistry, adjusting functional groups allows for the manipulation of these properties to improve pharmacokinetic profiles.

 

The pKa of a drug matters because it decides how much the drug will be charged or uncharged at different pH levels in the body. This charge affects how well the drug dissolves, passes through cell membranes, and interacts with target molecules. In pharmaceuticals, knowing the pKa helps anticipate how the drug will behave in the body, including how it’s absorbed, distributed, metabolized, and excreted. This information is vital for creating drug formulations, determining doses, and ensuring effectiveness.